Based on the two articles I have sầu read, these have the same concept in the case of antioxidant properties. One of them has mentioned, “IC50 value is a concentration of a sample required béo inhibit 50% of radical” (Molecules 2009, 14, 5349-53610). Và another one has mentioned, “the EC50 is a concentration of a sample required béo obtain a 1/2 antioxidant effect” (Food Chemistry Volume 138, Issue 1, 1 May 2013, Pages 414-420).Bạn đã xem: Ic50 là gì

Bài Viết: Ic50 là gì

The efficient concentration at different times EC50,t (mg extract mg-1 DPPH•) is the amount of the extracts in relation béo the amount of initial DPPH•, which calculated using the following equation:where IC50,t is the inhibitory concentration at different times, defined as the concentration of extract (mg mL-1) required to scavenge 50 % of DPPH• với t=0 is the initial concentration of DPPH• (mg mL-1).

Bạn đang xem: Ic50 là gì

The efficient concentration at different times EC50,t (mg extract mg-1 DPPH•) is the amount of the extracts in relation mập the amount of initial DPPH•, which calculated using the following equation:where IC50,t is the inhibitory concentration at different times, defined as the concentration of extract (mg mL-1) required mập scavenge 50 % of DPPH• và t=0 is the initial concentration of DPPH• (mg mL-1). The rest of this article is about IC50 (I for inhibition, for downward sloping dose-response curves). All the ideas can be applied mập stimulatory curves cùng EC50 (E for effective) as well.Can IC50 với EC50 be same of the drug? Suppose if we plot the dose response curve using % cytotoxiđô thị by MTT assay in case of death caused by virus, which is inhibited at various concentrations of drug? X axis involves vi khuẩn alone và virut in combination with various concentration of drug và Y axis has % cytotoxithành phố. How bự calculate IC50 cùng EC50 from this graph?in order mập evaluate the antioxidant activity in my plant extract i did the DPPH essay. i want mập express my result but i’m confused because the effective concentration at which the DPPH radical was scavenged by một nửa is mentioned as EC50 in some articles và IC50 in others.What is the meaning of IC 50 value in case of DPPH không tính phí radical scavenging method với how it is related with the antioxidant activity???I prepared Trolox working solution at different concentration – 0, 200, 500..800 uM in 10 ml abs MeOH. DPPH was used as antioxidant assay.For antioxidant activity procedure, 0.15 ml of each Trolox working solution was added bự 2.85 ml DPPH assay. Absorbance (515 nm) vs Trolox conc. gave linear correlation (R2=0.96776).

Working solution of sample was prepared by dissolving 20 mg dried sample in 10 ml abs MeOH, then 0.15 ml of it was mixed with 2.85 DPPH assay. Let say I got a certain absorbance value corresponding Khủng 200 uM Trolox, how could I biến hóa it to Trolox equivalent / (g-dry mass)? I confused whether Khủng multiply the denominator with 10 ml or 0.15 ml.

Thank you.I am testing antioxidants with the use of DPPH. Can anyone explain mập me whether béo use methanol/ethanol, how much of each concentrations I should use or how lớn work it out và what would be the best intervals etc.?I am a little confused as all literature varies in different ranges, so basically an easy protocol to follow would be of great help. Also, what else should I test for to validate antioxidant activity béo go alongside the DPPH?What is the significance of IC50 value when the drug is exogenously administered to an animal tissue?I have data for antioxidant activity on Excel sheet với I draw scatter chart with the equation: y = 1.3534 x + 0.58, với R² = 0.9927. I want Khủng calculate the IC50 for inhibition %. Someone can help me mập figure out how lớn calculate IC50 value?In addition, some of my colleagues use a function called “Growth” in Excel mập calculate IC50 by just by selection inhibition data column với then choose Growth from Insert Function (fx = ) với obtaining the result. Is it a correct way? i have sầu completed DPPH assay with Trolox as a standard. How bởi vì i express my results in Trolox equivalent/g dry weight?Effects of Solvents on the In-vitro Antioxidant Activity of Dennettia tripetala G. Baker với Milicia excelsa (Welw.) C. Berg Root Extracts

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